Difference between revisions of "Part:BBa K801081:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801081 short</partinfo>
 
<partinfo>BBa_K801081 short</partinfo>
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<partinfo>BBa_K801081 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K801081 SequenceAndFeatures</partinfo>
  
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Based on part BBa_K801080 (Prepro-Thaumatin).
 +
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
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<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
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 +
<br>'''Abbreviations:'''
 +
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
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<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
  
 
===Design Notes===
 
===Design Notes===
−
-
 
  
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'''Related BioBrick:'''
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<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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* Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K801080 BBa_K801080: Preprothaumatin]
  
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'''Cloning details:'''<br>
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* Designed in RFC10
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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'''Quality control measures:'''<br>
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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* Part was totally sequenced
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'''Backbone:'''<br>
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* Backbone name: pSB1C3
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<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--*Copynumber: low/medium/high-->
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'''Protein coding:'''<br>
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* Protein: Preprothaumatin [Nucleotide x to x]
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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* The protein encoded is posttranslationally modified: Pre- and Pro-sequence are split off
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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'''Enzymatic activity:'''
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* none
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'''Cytotoxicity:'''<br>
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* none
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'''Safety notes:'''<br>
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* none
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<!--Known and anticipated security issues: none/other.-->
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'''Intellectual property:'''
 +
<!--Information on patent situation.-->
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<!--Intellectual property claims made by the authors.-->
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'''Corresponding part author/authors:'''
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<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
−
Based on part BBa_K801080 (Prepro-Thaumatin).
+
'''Source:'''<br>
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<!--*Commercial system: plasmid name, system name, company name-->
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<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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<!--*Preexisting BioBrick ?Bba_number?-->
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<!--*cDNA Clone: ?clone_name?, ?company_name?-->
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<!--*Synthesized by ?company_name?.-->
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<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
 
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'''Organism:'''<br>
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<!--*Genesequence derived from ''?organism_name?''-->
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<!--*Codonoptimized for ''?organism_name?''-->
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<!--*Designed for the following Chassis: ''?organism-name?''-->
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<!--*Statement about functionality in other chassis.-->
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===References===
 
===References===
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<!-- Here you find templates to insert references to literature and different databases-->
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'''Literature references:'''<br>
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<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
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'''Database references:'''<br>
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<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
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<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
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<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
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<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
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<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 +
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 07:06, 27 September 2012

Prepro-Thaumatin expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205



Based on part BBa_K801080 (Prepro-Thaumatin).


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Cloning details:

  • Designed in RFC10

Quality control measures:

  • Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3

Protein coding:

  • Protein: Preprothaumatin [Nucleotide x to x]
  • The protein encoded is posttranslationally modified: Pre- and Pro-sequence are split off

Enzymatic activity:

  • none

Cytotoxicity:

  • none

Safety notes:

  • none

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: