Difference between revisions of "Part:BBa K838000"
Line 5: Line 5:
  
 
The lov domain clashes with the TRP repressor domain and prevents binding before the protein is exposed to blue light. After light activation the Lov domain goes through a comformational change and the TRP repressor domain of the protein can bind DNA.  
 
The lov domain clashes with the TRP repressor domain and prevents binding before the protein is exposed to blue light. After light activation the Lov domain goes through a comformational change and the TRP repressor domain of the protein can bind DNA.  
 +
 +
== Source ==
 +
 +
This part is an improvement on [[Part:BBa_K838000]] (LovTAP, a light-sensitive repressor expressed in bacteria).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:20, 27 September 2012

Mammalian LovTAP-VP16

LovTAP-VP16 is a light-sensitive protein that binds to DNA. The protein is a fusion between a Lov light activated domain, a TRP repressor, an NLS/ linker domain and a VP16 activator domain. As it contains a VP16 activational domain, it's able to act as a transcriptional activator when it is bound to a TRP promoter site.

The lov domain clashes with the TRP repressor domain and prevents binding before the protein is exposed to blue light. After light activation the Lov domain goes through a comformational change and the TRP repressor domain of the protein can bind DNA.

Source

This part is an improvement on Part:BBa_K838000 (LovTAP, a light-sensitive repressor expressed in bacteria).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 274
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 772
    Illegal XhoI site found at 817
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 274
  • 1000
    COMPATIBLE WITH RFC[1000]