Difference between revisions of "Part:BBa K953000"
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<i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then  complex with bacteriophytochrome (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As <i>E. coli</i> does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.
 
<i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then  complex with bacteriophytochrome (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As <i>E. coli</i> does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.
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<center>http://25.media.tumblr.com/tumblr_mazgigGnrs1rg4kjpo1_500.jpg</center>
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<center><i>Pellets of cells containing induced heme oxygenase, uninducded heme oxygenase and lacking heme oxygenase</i></center>
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Revision as of 02:00, 27 September 2012

Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome (Part BBa_K953001 or Part BBa_K953002). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.


http://25.media.tumblr.com/tumblr_mazgigGnrs1rg4kjpo1_500.jpg
Pellets of cells containing induced heme oxygenase, uninducded heme oxygenase and lacking heme oxygenase



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]