Difference between revisions of "Part:BBa K797003:Experience"
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The result shows that the expression of ''kil'' makes no effect on suvival of ''E.coli''. | The result shows that the expression of ''kil'' makes no effect on suvival of ''E.coli''. | ||
| − | [[Image: | + | [[Image:Kill_assay.png|thumb|left|300px|Fig.2-2 the result of evaluation of kil protein. Vertical axis means the value of OD 600. |
and horizontal axis means incuvation time.]]<br clear="both" /> | and horizontal axis means incuvation time.]]<br clear="both" /> | ||
Revision as of 01:09, 27 September 2012
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Applications of BBa_K797003
To check whether the expression of kil affects the growth of cells or not, we used the construct, lacp-RBS-Kil-double terminator. We did the experiment as this protocol, and we got the following data. Protocol
- Grow cells at 37 degrees for 18h.
- Dilute it until OD600=0.1.
- Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG.
- Incubate the cultures at 30 degrees and measure OD600 of them every 30 min
The result shows that the expression of kil makes no effect on suvival of E.coli.
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