Difference between revisions of "Part:BBa K808013"
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<partinfo>BBa_K808013 short</partinfo> | <partinfo>BBa_K808013 short</partinfo> | ||
| − | - | + | [[Image:Tph3.png.png|300px|thumb|right|Figure 1. '''Homology modelling of TphA3'''. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.]]]] |
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| + | ''TphA3'' is coding for the small terephthalate 1,2-dioxygenase subunit from ''Comamonas testosteroni KF-1''. TphA3 forms together with TphA1 and TphA2 the terephthalic acid 1,2-dioxygenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The monomer of TphA3 consists of 155 amino acids and has a molar mass of 17.36 kDa. Gel permeation chromatography shows that TphA3 has a homotrimeric structure and a mass of 52.08 kDa. | |
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| + | [[Image:TphA3.JPG|550px|thumb|left|Figure 2. '''GPC analysis of TphA3'''. The Peak of TphA3 has a retention time of 33 minutes. This retention time equates a molar mass of 52 kDa, approximately.[[http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Metabolism#Gel_permeation_chromatography click here.]]]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K808013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K808013 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K808013 parameters</partinfo> | <partinfo>BBa_K808013 parameters</partinfo> | ||
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| + | ==References== | ||
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| + | * Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832. | ||
| + | *Fukuhara, Y., K. Inakazu, et al. (2010). "Characterization of the isophthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 76(2): 519-527. | ||
| + | *Kamimura, N., T. Aoyama, et al. (2010). "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene." Appl Environ Microbiol 76(24): 8093-8101. | ||
| + | *Schläfli HR, Weiss MA, Leisinger T, Cook AM. (1994)"Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component." J Bacteriol. 1994 Nov;176(21):6644-52. | ||
Latest revision as of 01:01, 27 September 2012
tphA3: Catalyzes together with tphA2 TPA to DCD
Figure 1. Homology modelling of TphA3. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.
TphA3 is coding for the small terephthalate 1,2-dioxygenase subunit from Comamonas testosteroni KF-1. TphA3 forms together with TphA1 and TphA2 the terephthalic acid 1,2-dioxygenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation
Usage and Biology
The monomer of TphA3 consists of 155 amino acids and has a molar mass of 17.36 kDa. Gel permeation chromatography shows that TphA3 has a homotrimeric structure and a mass of 52.08 kDa.
Figure 2. GPC analysis of TphA3. The Peak of TphA3 has a retention time of 33 minutes. This retention time equates a molar mass of 52 kDa, approximately.http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Metabolism#Gel_permeation_chromatography click here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832.
- Fukuhara, Y., K. Inakazu, et al. (2010). "Characterization of the isophthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 76(2): 519-527.
- Kamimura, N., T. Aoyama, et al. (2010). "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene." Appl Environ Microbiol 76(24): 8093-8101.
- Schläfli HR, Weiss MA, Leisinger T, Cook AM. (1994)"Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component." J Bacteriol. 1994 Nov;176(21):6644-52.