Difference between revisions of "Part:BBa K797003:Experience"
Grassfield (Talk | contribs) (→Applications of BBa_K797003) |
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# Grow cells at 37 degrees for 18h. | # Grow cells at 37 degrees for 18h. | ||
# Dilute it until OD600=0.1. | # Dilute it until OD600=0.1. | ||
| + | # Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG. | ||
| + | # Incubate the cultures at 30 degrees and measure OD600 of them every 30 min | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 00:58, 27 September 2012
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Applications of BBa_K797003
To check whether the expression of kil affects the growth of cells or not, we used the construct, lacp-RBS-Kil-double terminator. We did the experiment as this protocol, and we got the following data. Protocol
- Grow cells at 37 degrees for 18h.
- Dilute it until OD600=0.1.
- Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG.
- Incubate the cultures at 30 degrees and measure OD600 of them every 30 min
User Reviews
UNIQ764a3c4d00c722d8-partinfo-00000000-QINU UNIQ764a3c4d00c722d8-partinfo-00000001-QINU