Difference between revisions of "Part:BBa K775000"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K775000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K775000 SequenceAndFeatures</partinfo>
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GFP intensity of S. cerevisiae cells transformed with ''FUS1pr-EGFP'' at t=5.10 after induction with alpha pheromone. Please also see our page: [http://2012.igem.org/Team:TU-Delft/part2 Reporter]
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[[Image:FUSGFP_Growth_Intensity_Div.png|960px]]
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GFP intensity and growth curves of S. cerevisiae transformed with ''FUS1pr-EGFP'' after induction with alpha pheromone
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[[Image:FUSGFP_Div_Time.png|960px]]
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 00:38, 27 September 2012

RI7-GPR109A Niacin chimeric GPCR

BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 582
    Illegal BamHI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 478