Difference between revisions of "Part:BBa K899005"

 
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<partinfo>BBa_K899005 short</partinfo>
 
<partinfo>BBa_K899005 short</partinfo>
  
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Heat inducible promoter K33801 regulating the expression of sucrose sucrose fructosyltransferase (SST) gene and a toxin-antitoxin (TA) system compatible with part BBa_K899006. The TA system in this part contains ccdB as a toxin and MazE as antitoxin.
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Heat inducible promoter K33801 regulating the expression of sucrose sucrose fructosyltransferase (SST) gene and a toxin-antitoxin (TA) system compatible with part BBa_K899006. The TA system in this part contains ccdB as a toxin and MazE as antitoxin. It should be noted that since we put the antitoxin last (and the antitoxin degrades faster than the toxin) there should be a gtg start codon for the toxins in this part and BBa_K899006 and a ATG startcodon in front of the antitoxins. This should equalize the relationship between antitoxin and toxin molecules.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 00:28, 27 September 2012

heat inducible SST with TA system compatible with BBa_K899006

Heat inducible promoter K33801 regulating the expression of sucrose sucrose fructosyltransferase (SST) gene and a toxin-antitoxin (TA) system compatible with part BBa_K899006. The TA system in this part contains ccdB as a toxin and MazE as antitoxin. It should be noted that since we put the antitoxin last (and the antitoxin degrades faster than the toxin) there should be a gtg start codon for the toxins in this part and BBa_K899006 and a ATG startcodon in front of the antitoxins. This should equalize the relationship between antitoxin and toxin molecules.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1930
    Illegal BamHI site found at 223
    Illegal BamHI site found at 713
    Illegal BamHI site found at 2402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 174
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2239