Difference between revisions of "Part:BBa K808010"

Revision as of 00:17, 27 September 2012

tphB: reaction from DCD to protocatechuate

Homology modelling of TphB. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphB click here.

TphB is coding for the decarboxylating cis-dihydrodiol dehydrogenase from Comamonas testosteroni Kf-1. It catalyzes the second reaction in the terephthalic acid degration - the decarboxylation of DCD to protocatechuate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 155
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 202
Illegal NgoMIV site found at 877
Illegal AgeI site found at 939
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 76

References

Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832.
Fukuhara, Y., K. Inakazu, et al. (2010). "Characterization of the isophthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 76(2): 519-527.
Kamimura, N., T. Aoyama, et al. (2010). "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene." Appl Environ Microbiol 76(24): 8093-8101.
Schläfli HR, Weiss MA, Leisinger T, Cook AM. (1994)"Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component." J Bacteriol. 1994 Nov;176(21):6644-52.
Bains, J., J.E. Wulff, and M.J. Boulanger, Investigating Terephthalate Biodegradation: Structural Characterization of a Putative Decarboxylating cis-Dihydrodiol Dehydrogenase. J Mol Biol, 2012.

@@ Line 3: / Line 3: @@
 [[Image:TPHB.full.png|300px|thumb|right|'''Homology modelling of TphB'''. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphB click here.]]]]
-tphB is coding for the dihydrodiol decarboxylase from ''Comamonas testosteroni Kf-1''. It´s catalyze the second reaction in the terephthalic acid degration.
+''TphB'' is coding for the  decarboxylating  ''cis''-dihydrodiol dehydrogenase from ''Comamonas testosteroni Kf-1''. It catalyzes the second reaction in the terephthalic acid degration - the decarboxylation of DCD to protocatechuate.
-===Usage and Biology===