Difference between revisions of "Part:BBa K812032"
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mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used in pSB1C3. | mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used in pSB1C3. | ||
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| + | The caracterisation was performed in pCS2+ plasmid<html>(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>)</html>. | ||
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Revision as of 23:58, 26 September 2012
mCFP with kozak sequence for eukaryotic expression
mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used in pSB1C3.
The caracterisation was performed in pCS2+ plasmid(K812000).
Tadpole 1 is 2 days (stage 36-38), the expression of CFP is localized in intestine. Tadpole 2 is 4 days (stage 45-46), the expression of CFP is localized in Branchial basket and intestine
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]