Difference between revisions of "Part:BBa K896008:Experience"
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[[Image:flu 200.jpg]] | [[Image:flu 200.jpg]] | ||
| − | Before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au. | + | *Before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au. |
[[Image:Cd 15.jpg]] | [[Image:Cd 15.jpg]] | ||
| − | This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion. | + | *This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion. |
===Applications of BBa_K896008=== | ===Applications of BBa_K896008=== | ||
Revision as of 23:40, 26 September 2012
- Before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au.
- This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion.
Applications of BBa_K896008
It can be used to detect cadmium ion when ligating with GFP.
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UNIQf03e78f7b11951a7-partinfo-00000000-QINU UNIQf03e78f7b11951a7-partinfo-00000001-QINU