Difference between revisions of "Part:BBa K864440"
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<partinfo>BBa_K864440 short</partinfo>
 
<partinfo>BBa_K864440 short</partinfo>
  
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The natural small RNA spot42 taken from the E-coli MG1655. Small RNA is belived to interact in many metabolic pathways. The spot42 scaffold interacts with HFQ-proteins. It is in many cases shown to bind into the rbs area of the mRNA and thereby blocking translation initiation.  ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 )
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The natural small RNA spot42 taken from the E-coli MG1655. Small RNA is belived to interact in metabolic pathways. The spot42 scaffold recruits Hfq proteins. In some cases it has been shown to bind to the ribosomal binding site of the mRNA and thereby blocking translation.  ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 )
  
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We wanted to use this sRNA for gene downregulation on translational level, in this case block the ribosome from binding to the rbs area.  
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We wanted to use this sRNA for gene down regulation on translational level, in this case block the ribosome from binding to the rbs area.  
  
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You can use this natural scaffold to make your own sRNA, inhibiting translation on a gene of your choice.  
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You can use this natural scaffold to make your own sRNA that could inhibit translation of a gene in question.  
 
The sRNA is coupled to a strong promotor, J23101.
 
The sRNA is coupled to a strong promotor, J23101.
  

Revision as of 23:33, 26 September 2012

Small RNA spot42

The natural small RNA spot42 taken from the E-coli MG1655. Small RNA is belived to interact in metabolic pathways. The spot42 scaffold recruits Hfq proteins. In some cases it has been shown to bind to the ribosomal binding site of the mRNA and thereby blocking translation. ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 )

We wanted to use this sRNA for gene down regulation on translational level, in this case block the ribosome from binding to the rbs area.

You can use this natural scaffold to make your own sRNA that could inhibit translation of a gene in question. The sRNA is coupled to a strong promotor, J23101.

Reference

Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli Vandana Sharma, Asami Yamamura, and Yohei Yokobayashi ACS Synthetic Biology 2012 1 (1), 6-13


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]