Difference between revisions of "Part:BBa K775000"

Revision as of 23:19, 26 September 2012

RI7-GPR109A Niacin chimeric GPCR

BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 582
Illegal BamHI site found at 113
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 478

GFP intensity of S. cerevisiae cells transformed with FUS1pr-EGFP at t=5.10 after induction with alpha pheromone. Please also see our page: [http://2012.igem.org/Team:TU-Delft/part2 Reporter]

GFP intensity and growth curves of S. cerevisiae transformed with FUS1pr-EGFP after induction with alpha pheromone

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 __NOTOC__
 <partinfo>BBa_K775000 short</partinfo>
@@ Line 11: / Line 10: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K775000 SequenceAndFeatures</partinfo>
+<br>
+GFP intensity of S. cerevisiae cells transformed with ''FUS1pr-EGFP'' at t=5.10 after induction with alpha pheromone. Please also see our page: [http://2012.igem.org/Team:TU-Delft/part2 Reporter]
+<br>
+[[Image:FUSGFP_Growth_Intensity_Div.png|960px]]
+<br>
+GFP intensity and growth curves of S. cerevisiae transformed with ''FUS1pr-EGFP'' after induction with alpha pheromone
+<br>
+[[Image:FUSGFP_Div_Time.png|960px]]
 <!-- Uncomment this to enable Functional Parameter display