Difference between revisions of "Part:BBa K801075"

Revision as of 22:51, 26 September 2012

CaDXMT1 expression cassette for yeast

Yeast expression cassette for CaDXMT1 (BBa_K801072) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012)

This BioBrick is the generator for the enzyme 3,7-dimethylxanthine N-methyltransferase 1 (CaDXMT1) (= caffeine synthase), i.e. the last enzyme envolved in caffeine biosynthesis. It is regulated by the constitutive promoter Tef2, which is also used at the expression cassette of CaXMT1. The used terminator is Adh1. The choice of the strong Tef1 promoter was made, because the in vivo biosynthesis of caffeine is an irreversible reaction, which is ensured by continously increasing Km and vmax values of the single enzymes. As soon as a certain amount of theobromine is available, the caffeine synthesis can go on. The choice for this promoter was made to prevent metabolic stress reactions and because of the required irreversibility of the caffeine production (see above).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1650
Illegal BglII site found at 1746
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K801075 short</partinfo>
 Yeast expression cassette for CaDXMT1 (BBa_K801072) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012)
+This BioBrick is the generator for the enzyme 3,7-dimethylxanthine N-methyltransferase 1 (CaDXMT1) (= caffeine synthase), i.e. the last enzyme envolved in caffeine biosynthesis. It is regulated by the constitutive promoter Tef2, which is also used at the expression cassette of [https://parts.igem.org/Part:BBa_K801073 CaXMT1]. The used terminator is Adh1. The choice of the strong Tef1 promoter was made, because the in vivo biosynthesis of caffeine is an irreversible reaction, which is ensured by continously increasing Km and vmax values of the single enzymes. As soon as a certain amount of theobromine is available, the caffeine synthesis can go on. The choice for this promoter was made to prevent metabolic stress reactions and because of the required irreversibility of the caffeine production (see above).
 <!-- Add more about the biology of this part here