Difference between revisions of "Part:BBa K782068"
UrosZupancic (Talk | contribs) |
|||
| (4 intermediate revisions by 2 users not shown) | |||
| Line 3: | Line 3: | ||
* TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011). | * TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011). | ||
| + | * DNA binding sites for individual TAL effectors are indicated with square brackets [ ]. | ||
| + | |||
==Introduction== | ==Introduction== | ||
| Line 17: | Line 19: | ||
'''Figure 1.''' Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD. | '''Figure 1.''' Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD. | ||
| + | |||
| + | |||
| + | * Binding sites for TAL effectors were ordered from IDT. | ||
==Characterization== | ==Characterization== | ||
| + | Specific TAL binding sites were further characterized with different reporter constructs. | ||
| − | Results: | + | '''Results:''' |
| − | * | + | * [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782025 BBa_K782025] |
| Line 31: | Line 37: | ||
Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53. | Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53. | ||
| − | Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698 | + | Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698. |
Latest revision as of 22:51, 26 September 2012
4x[NicTAL]+4x[TALD] operator
- TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
- DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
Introduction
Our construct contains four specific binding sites for NicTAL12 and TALD.
Single binding sequence for NicTAL12 is: TCTATCAATGATAGA
Single binding sequence for TALD is: TCGTCCAATAGCTTCTC
Figure 1. Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD.
- Binding sites for TAL effectors were ordered from IDT.
Characterization
Specific TAL binding sites were further characterized with different reporter constructs.
Results:
References
Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
Illegal AgeI site found at 235 - 1000COMPATIBLE WITH RFC[1000]