Difference between revisions of "Part:BBa K782068"

 
 
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<partinfo>BBa_K782068 short</partinfo>
 
<partinfo>BBa_K782068 short</partinfo>
  
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* TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
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* DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
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==Introduction==
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Our construct contains four specific binding sites for [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782007 NicTAL12] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782005 TALD].
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Single binding sequence for NicTAL12 is: TCTATCAATGATAGA
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Single binding sequence for TALD is: TCGTCCAATAGCTTCTC
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[[Image:4xNic+4xD.png‎]]
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'''Figure 1.''' Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD.
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* Binding sites for TAL effectors were ordered from IDT.
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==Characterization==
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Specific TAL binding sites were further characterized with different reporter constructs.
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'''Results:'''
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* [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782025 BBa_K782025]
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==References==
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Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.
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Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:51, 26 September 2012

4x[NicTAL]+4x[TALD] operator

  • TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
  • DNA binding sites for individual TAL effectors are indicated with square brackets [ ].


Introduction

Our construct contains four specific binding sites for NicTAL12 and TALD.

Single binding sequence for NicTAL12 is: TCTATCAATGATAGA

Single binding sequence for TALD is: TCGTCCAATAGCTTCTC


4xNic+4xD.png

Figure 1. Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD.


  • Binding sites for TAL effectors were ordered from IDT.


Characterization

Specific TAL binding sites were further characterized with different reporter constructs.

Results:


References

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
    Illegal AgeI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]