Difference between revisions of "Part:BBa K801092:Design"

Revision as of 22:49, 26 September 2012

4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1092
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1659
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1291

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/609339/

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase

Abbreviations: 4CL = 4-coumarate-coenzyme A Ligase

Design Notes

Related BioBrick:

Quality control measures:
Part was totally sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Protein coding:
Protein: 4-coumarate-coenzyme A ligase

Enzymatic activity:
EC-number: 6.2.1.12

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Arabidopsis thaliana

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 GenBank: Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q42524 Uniprot: 4-coumarate--CoA ligase 1]

@@ Line 11: / Line 11: @@
 PMID: 15932991
 <br>'''Keywords:'''
 Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase