Difference between revisions of "Part:BBa K801073"
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Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).
 
Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).
  
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This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1). It is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator is Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).
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This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1)and part of the caffeine synthesis pathway. It is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator is Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).
  
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This BioBrick is a part of the caffeine synthesis pathway.
 
  
 
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Revision as of 22:48, 26 September 2012

CaXMT1 expression cassette for yeast

Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).

This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1)and part of the caffeine synthesis pathway. It is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator is Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
  • 1000
    COMPATIBLE WITH RFC[1000]