Difference between revisions of "Part:BBa K909013"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K909013 short</partinfo>
 
<partinfo>BBa_K909013 short</partinfo>
  
The promoter is derived from the PR (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions were replaced by the operators cI OR1, cI OR2 and TetO1.
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The promoter is derived from the P<sub>R</sub> (lambda phage) promoter. The -35 and -10 regions as well as the cI operator sites are maintained while the sequence downstream of the -10 region is replaced by the tetR operator TetO<sub>1</sub>.
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This part serves as a '''biological NOR-gate'''.
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[[Image:CI_tet.png]]
  
<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 22:47, 26 September 2012

PR promoter with multiple operator sites: cI OR1, cI OR2 and TetO1

The promoter is derived from the PR (lambda phage) promoter. The -35 and -10 regions as well as the cI operator sites are maintained while the sequence downstream of the -10 region is replaced by the tetR operator TetO1.

This part serves as a biological NOR-gate.

CI tet.png

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]