Difference between revisions of "Part:BBa K801092:Design"
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'''Database references:'''<br>
 
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[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 '''GenBank''': Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]
 
[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 '''GenBank''': Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]
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[http://www.uniprot.org/uniprot/Q42524  '''Uniprot''': 4-coumarate--CoA ligase 1]-->
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[http://www.uniprot.org/uniprot/Q42524  '''Uniprot''': 4-coumarate--CoA ligase 1]
 
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Revision as of 22:47, 26 September 2012

4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1291


Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/609339/

PMID: 15932991
Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase


Abbreviations: 4CL = 4-coumarate-coenzyme A Ligase

Design Notes

Related BioBrick:

Quality control measures:
Part was totally sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Protein coding:
Protein: 4-coumarate-coenzyme A ligase

Enzymatic activity: EC-number: 6.2.1.12

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA


Organism:
Genesequence derived from Arabidopsis thaliana


References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 GenBank: Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q42524 Uniprot: 4-coumarate--CoA ligase 1]