Difference between revisions of "Part:BBa K801003:Design"
(→‎Design Notes)
Line 22: Line 22:
  
 
'''Related BioBrick:'''
 
'''Related BioBrick:'''
 +
* [[BBa_K801002]]
 +
* [[BBa_K801001]]
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
−
'''Cloning details:'''<br>
+
'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
  
−
'''Quality control measures:'''<br>
+
'''Quality control measures:'''<br> Test digestion using SpeI+PstI-HF
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
  
−
'''Backbone:'''<br>
+
'''Backbone:'''<br> Resistance: Amp
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->

Revision as of 22:39, 26 September 2012

pTUM103 yeast shuttle vector with pADH1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 776
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 4041
    Illegal SpeI site found at 777
    Illegal PstI site found at 791
    Illegal NotI site found at 42
    Illegal NotI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 777
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 777
    Illegal PstI site found at 791
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 149
    Illegal BsaI.rc site found at 2198
    Illegal BsaI.rc site found at 3144
    Illegal SapI site found at 1115
    Illegal SapI site found at 3296


All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible .


Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using SpeI+PstI-HF

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: