Difference between revisions of "Part:BBa K782079"
 
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<partinfo>BBa_K782079 short</partinfo>
 
<partinfo>BBa_K782079 short</partinfo>
  
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TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).
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* TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).
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* DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
  
 
==Introduction==
 
==Introduction==
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Results:  
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Results: Specific TAL [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782020 binding sites] were further characterized with other reporter constructs.
  
 
BFP was obtained from Evrogen.
 
BFP was obtained from Evrogen.
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==References==
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Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.
  
 
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Latest revision as of 22:36, 26 September 2012

10x[TALA] operator_CMV promoter_2xBFP:NLS

  • TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).
  • DNA binding sites for individual TAL effectors are indicated with square brackets [ ].

Introduction

Our construct contain TALA binding sites that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Abfp2.png

Figure 1: Schematic representation of the construct.


Characterization

Results: Specific TAL binding sites were further characterized with other reporter constructs.

BFP was obtained from Evrogen.

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 507
    Illegal AgeI site found at 12
    Illegal AgeI site found at 347
    Illegal AgeI site found at 372
    Illegal AgeI site found at 707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1396
    Illegal BsaI.rc site found at 2052
    Illegal BsaI.rc site found at 2757