Difference between revisions of "Part:BBa K782077"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K782077 short</partinfo>
 
<partinfo>BBa_K782077 short</partinfo>
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* DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
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==Introduction==
 
==Introduction==
  
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Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782011 NicTAL12] ([https://parts.igem.org/Part:BBa_K782007 sequence]) that are cloned upstream of CMV promoter. Downstream  of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
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Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782007 NicTAL12] ([https://parts.igem.org/Part:BBa_K782003 binding site's sequence]) that are cloned upstream of CMV promoter. Downstream  of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
  
 
[[Image:Nicbfp.png‎‎]]
 
[[Image:Nicbfp.png‎‎]]
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Results:  
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Results: Specific TAL [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782023 binding sites] were further characterized with other reporter constructs.
  
 
BFP was obtained from Evrogen.
 
BFP was obtained from Evrogen.
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==References==
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Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.
  
  

Latest revision as of 22:35, 26 September 2012

12x[NicTAL] operator_CMV promoter_BFP

  • DNA binding sites for individual TAL effectors are indicated with square brackets [ ].


Introduction

Our construct contain twelve binding sites for NicTAL12 (binding site's sequence) that are cloned upstream of CMV promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Nicbfp.png

Figure 1: Schematic representation of the construct.


Characterization

Results: Specific TAL binding sites were further characterized with other reporter constructs.

BFP was obtained from Evrogen.

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 16
    Illegal AgeI site found at 248
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1140
    Illegal BsaI.rc site found at 1796