Difference between revisions of "Part:BBa K909011"
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<partinfo>BBa_K909011 short</partinfo> | <partinfo>BBa_K909011 short</partinfo> | ||
| − | The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions was replaced by the | + | The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions was replaced by the operator regions TetO<sub>1</sub> from the tetR promoter on Transposon Tn10 and the operator regions LacO<sub>1</sub> and LacO<sub>2</sub> from the Lac operon. |
| + | This part serves as a '''biological NOR-gate'''. | ||
[[Image:tetlac.png]] | [[Image:tetlac.png]] | ||
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| − | <!-- | + | <!-- |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 22:32, 26 September 2012
Dual input promoter: PL promoter with two operator sites TetO1 and LacO1
The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions was replaced by the operator regions TetO1 from the tetR promoter on Transposon Tn10 and the operator regions LacO1 and LacO2 from the Lac operon.
This part serves as a biological NOR-gate.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]