Difference between revisions of "Part:BBa K801001:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
−
'''Related BioBrick:'''
+
'''Related BioBrick:''' [https://parts.igem.org/wiki/index.php?title=Part:BBa K801000: BBa K801001]
 +
*[https://parts.igem.org/wiki/index.php?title=Part:BBa K801002: BBa K801002]
 +
*[https://parts.igem.org/wiki/index.php?title=Part:BBa K801003: BBa K801003]
 +
 
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
−
'''Cloning details:'''<br>
+
'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
  
−
'''Quality control measures:'''<br>
+
'''Quality control measures:'''<br> Test digestion using SpeI+ PstI
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
  
−
'''Backbone:'''<br>
+
'''Backbone:'''<br> Resistance: Amp
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->

Revision as of 22:30, 26 September 2012

pTUM101 yeast shuttle vector with pTEF1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3771
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
    Illegal NotI site found at 42
    Illegal NotI site found at 514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 261
    Illegal BsaI.rc site found at 1928
    Illegal BsaI.rc site found at 2874
    Illegal SapI site found at 845
    Illegal SapI site found at 3026

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).


Keywords:


Abbreviations:

Design Notes

Related BioBrick: K801000: BBa K801001


Quality control measures:
Test digestion using SpeI+ PstI

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: