Difference between revisions of "Part:BBa K738002:Design"
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===Design Notes=== | ===Design Notes=== | ||
Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (''rne''131) gene to limit RNA degradation. | Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (''rne''131) gene to limit RNA degradation. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 22:24, 26 September 2012
Theophyline riboswitch regulated RNA Scaffold (clover vision 2)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.
Source
Clover version 2 was synthesized by Genescript.
References
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).