Difference between revisions of "Part:BBa K801090:Design"
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'''Source:'''<br> | '''Source:'''<br> | ||
<!--*Commercial system: plasmid name, system name, company name--> | <!--*Commercial system: plasmid name, system name, company name--> | ||
− | <!--*Plasmid: | + | <!--*Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA |
<!--*Preexisting BioBrick ?Bba_number?--> | <!--*Preexisting BioBrick ?Bba_number?--> | ||
<!--*cDNA Clone: ?clone_name?, ?company_name?--> | <!--*cDNA Clone: ?clone_name?, ?company_name?--> | ||
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'''Organism:'''<br> | '''Organism:'''<br> | ||
− | <!--*Genesequence derived from '' | + | <!--*Genesequence derived from ''Rhodosporidium toruloides''--> |
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
<!--*Statement about functionality in other chassis.--> | <!--*Statement about functionality in other chassis.--> | ||
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===References=== | ===References=== |
Revision as of 22:14, 26 September 2012
phenylalanine ammonia lyase (PAL) + yeast consensus sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2096
Illegal BamHI site found at 358
Illegal XhoI site found at 409
Illegal XhoI site found at 472
Illegal XhoI site found at 490
Illegal XhoI site found at 568
Illegal XhoI site found at 769
Illegal XhoI site found at 1012
Illegal XhoI site found at 1759 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 928
Illegal NgoMIV site found at 1174
Illegal NgoMIV site found at 1342
Illegal NgoMIV site found at 1485
Illegal NgoMIV site found at 1819 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 469
Illegal BsaI site found at 799
Illegal BsaI site found at 805
Illegal BsaI.rc site found at 1930
Illegal SapI site found at 759
Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
http://www.ncbi.nlm.nih.gov/nuccore/AX366866
PMID: 15932991
Keywords:
Abbreviations:
Design Notes
Related BioBrick:
Quality control measures:
Backbone:
Protein coding:
Enzymatic activity:
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
Organism:
References
Literature references:
Database references:
GenBank : [http://www.ncbi.nlm.nih.gov/nuccore/AX366866 accession no. AX366866]