Difference between revisions of "Part:BBa K801011:Design"
(→‎Design Notes)
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
−
'''Cloning details:'''<br>
+
'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25/RFC21
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
  
−
'''Quality control measures:'''<br>
+
'''Quality control measures:'''<br> Test digestion using XbaI+PstI-HF, EcorI+ SpeI-HF
 +
*Sequencing using primer VR and VF2
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
  
−
'''Backbone:'''<br>
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'''Backbone:'''<br> Backbone name: pSB1C3
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--none/EC-number ?.?.?.?-->
 
<!--none/EC-number ?.?.?.?-->
  
−
'''Cytotoxicity:'''<br>
+
'''Cytotoxicity:'''<br> none
 
<!--none/not known/cytotoxic for ''organism name''-->
 
<!--none/not known/cytotoxic for ''organism name''-->
  
−
'''Safety notes:'''<br>
+
'''Safety notes:'''<br> none
−
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
+
<!--Known and anticipated safety issues: none/health_risk/environmental_risk/other_risk-->
 
<!--Known and anticipated security issues: none/other.-->
 
<!--Known and anticipated security issues: none/other.-->
  

Revision as of 22:05, 26 September 2012

TEF1 yeast terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.



Extracted from genomic DNA of S. cerevisiae using PCR.


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using XbaI+PstI-HF, EcorI+ SpeI-HF

  • Sequencing using primer VR and VF2

Backbone:
Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: