Difference between revisions of "Part:BBa K778011"
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E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | ||
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). | fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). | ||
− | Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] | + | Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] |
Revision as of 22:00, 26 September 2012
hycAp-GFPgenerator-plac-FhlA363generator
You can "enhance" and "see" H2 production with this device.
hycAp-GFPgenerator-plac-FhlA363generator
GFP is a reportor of H2 production (strictly speaking,reporter of hycAp activation Part:BBa K778000 (hycAp is the promotor of formate hydrogen lyase of genome)).
FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2545
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 828