Difference between revisions of "Part:BBa K801010:Design"
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===Source=== | ===Source=== | ||
− | '''Source:'''<br> | + | '''Source:'''<br> PCR from genomic DNA of ''Saccharomyces cerevisiae'' |
<!--*Commercial system: plasmid name, system name, company name--> | <!--*Commercial system: plasmid name, system name, company name--> | ||
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | ||
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<!--*cDNA Clone: ?clone_name?, ?company_name?--> | <!--*cDNA Clone: ?clone_name?, ?company_name?--> | ||
<!--*Synthesized by ?company_name?.--> | <!--*Synthesized by ?company_name?.--> | ||
− | + | * Forward Primer: VF2 | |
+ | * Reverse Primer: VR | ||
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>--> | <!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>--> | <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
− | '''Organism:'''<br> | + | '''Organism:'''<br> Developed for ''Saccharomyces cerevisiae'' |
<!--*Genesequence derived from ''?organism_name?''--> | <!--*Genesequence derived from ''?organism_name?''--> | ||
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
<!--*Statement about functionality in other chassis.--> | <!--*Statement about functionality in other chassis.--> | ||
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===References=== | ===References=== |
Revision as of 21:53, 26 September 2012
TEF2 constitutive yeast promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 137
- 1000COMPATIBLE WITH RFC[1000]
Extracted from genomic DNA of S. cerevisiae using PCR.
Keywords:
Abbreviations:
Design Notes
Related BioBrick:BBa_K165037
Quality control measures:
Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF
Backbone:
Backbone name: pSB1C3, Resistance: Cp
Protein coding:
Enzymatic activity:
Cytotoxicity:
none
Safety notes:
none
Intellectual property:
Corresponding part author/authors:
Source
Source:
PCR from genomic DNA of Saccharomyces cerevisiae
- Forward Primer: VF2
- Reverse Primer: VR
Organism:
Developed for Saccharomyces cerevisiae
References
Literature references:
Database references: