Difference between revisions of "Part:BBa K863103:Design"

 
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<partinfo>BBa_K863103 short</partinfo>
 
<partinfo>BBa_K863103 short</partinfo>
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===Design Notes===
 
===Design Notes===
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CBDcex fused to a his-tagged GFP in a Freiburg-manner
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This Biobrick is the result of a Standard 25 assembly of the BioBricks <partinfo>BBa_K863102</partinfo> and <partinfo>BBa_K863121</partinfo> and was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) with a fused GFP as a reporter protein under the control of a T7-promoter <partinfo>BBa_K525998</partinfo>. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP.
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===Source===
 
===Source===
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The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and [https://parts.igem.org/Part:BBa_K863121 BBa_K863121] as template for the GFP-fusion protein.
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Cloning-vector and [https://parts.igem.org/Part:BBa_K863121 BBa_K863121]
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===References===
 
===References===

Latest revision as of 20:53, 26 September 2012

Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1103
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1014


Design Notes

This Biobrick is the result of a Standard 25 assembly of the BioBricks BBa_K863102 and BBa_K863121 and was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) with a fused GFP as a reporter protein under the control of a T7-promoter BBa_K525998. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP.

Source

The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and BBa_K863121 as template for the GFP-fusion protein.

References