Difference between revisions of "Part:BBa K778008"

Revision as of 20:10, 26 September 2012

FhlA-E363G generator (pLac-RBS-fhlA363-d.term)

plac-rbs-fhlA363-d.term

FhlA-E363G generator (pLac)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).

This biobrick part can be used to overexpress FhlA-E363G with IPTG induction for enhanced hydrogen production.

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2178
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1457
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K778008 short</partinfo>
 plac-rbs-fhlA363-d.term
+FhlA-E363G generator (pLac)
+FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .
+E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
+fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
+This biobrick part can be used to overexpress FhlA-E363G with IPTG induction for enhanced hydrogen production.
+Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
 <!-- Add more about the biology of this part here