Difference between revisions of "Part:BBa K778007"
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E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
 
E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
  
−
You can overexpress FhlA with IPTG induction for enhanced hydrogen production.  
+
This biobrick part can be used to overexpress FhlA with IPTG induction for enhanced hydrogen production.  
  
 
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
 
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]

Revision as of 20:01, 26 September 2012

FhlA-generator (pLac-RBS-fhlA-d.term)

pLac-RBS-fhlA-d.term

FhlA generator (pLac)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

This biobrick part can be used to overexpress FhlA with IPTG induction for enhanced hydrogen production.

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1457
  • 1000
    COMPATIBLE WITH RFC[1000]