Difference between revisions of "Part:BBa K733004:Design"
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We obtain this part from the genomic DNA of ''Bacillus subtilis 168'' via PCR. | We obtain this part from the genomic DNA of ''Bacillus subtilis 168'' via PCR. | ||
| − | Forward Primer: 5’- | + | Forward Primer: 5’- AAAGGGGGAAATAATCATTTGATTGTGAAACGC-3’ (Prefix not shown) |
Reverse Primer: 5’-AATATGTCTAAAAATCAATGAGTGC-3’ (Suffix not shown) | Reverse Primer: 5’-AATATGTCTAAAAATCAATGAGTGC-3’ (Suffix not shown) | ||
===References=== | ===References=== | ||
Latest revision as of 19:52, 26 September 2012
RBS+ydcE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To easily building an antitoxin expression device, forward primer containing consensus RBS sequence in B. subtilis is designed, aiming at adding RBS directly to the upstream of ydcE gene through PCR.
Source
We obtain this part from the genomic DNA of Bacillus subtilis 168 via PCR.
Forward Primer: 5’- AAAGGGGGAAATAATCATTTGATTGTGAAACGC-3’ (Prefix not shown)
Reverse Primer: 5’-AATATGTCTAAAAATCAATGAGTGC-3’ (Suffix not shown)