Difference between revisions of "Part:BBa K801002:Design"

Revision as of 19:37, 26 September 2012

pTUM102 yeast shuttle vector with pTEF2 promoter

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 36
Illegal suffix found at 705
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 3970
Illegal SpeI site found at 706
Illegal PstI site found at 720
Illegal NotI site found at 42
Illegal NotI site found at 713
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 36
Illegal suffix found at 706
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 36
Plasmid lacks a suffix.
Illegal XbaI site found at 51
Illegal SpeI site found at 706
Illegal PstI site found at 720
Illegal NgoMIV site found at 194
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2127
Illegal BsaI.rc site found at 3073
Illegal SapI site found at 1044
Illegal SapI site found at 3225

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible.

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Keywords:

pTUM, pYES2, TEF2-Promoter

Abbreviations:

TEF2

Design Notes

Related BioBrick: pTUM100, pTUM101, pTUM103, pTUM104

Quality control measures:
Testdigestion using XbaI+PstI-HF, and EcorI-HF+SpeI-HF

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity: none

Cytotoxicity:

Safety notes:
health_risk

Intellectual property:

Corresponding part author/authors:

Source

Source:
pYES2, pYES2 Vector kit, company: Invitrogen

Organism:
Designed for E.coli, Yeast

References

Literature references:

Database references:

@@ Line 12: / Line 12: @@
 <br>'''Keywords:'''
+* pTUM, pYES2, TEF2-Promoter
 <!--These keywords are necessary to find your part using a fulltext sarch.-->
 <!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
 <br>'''Abbreviations:'''
+* TEF2
 <!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
 <!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
@@ Line 21: / Line 23: @@
 ===Design Notes===
-'''Related BioBrick:'''
+'''Related BioBrick:''' pTUM100, pTUM101, pTUM103, pTUM104
 <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
-'''Cloning details:'''<br>
+'''Cloning details:'''<br> Designed in RFC10
 <!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
-'''Quality control measures:'''<br>
+'''Quality control measures:'''<br> Testdigestion using XbaI+PstI-HF, and EcorI-HF+SpeI-HF
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 <!--*Part was partly sequenced/Part was totally sequenced-->
-'''Backbone:'''<br>
+'''Backbone:'''<br> Resistance: Amp
 <!--*Backbone name: pSB1C3'/?backbone_name?-->
 <!--*Resistance: Amp/Cp/Kan/Tet-->
 <!--*Copynumber: low/medium/high-->
 '''Protein coding:'''<br>
 <!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
 <!--*The protein has the amino acid replacements ???99??? to ???99???.-->
@@ Line 46: / Line 48: @@
 <!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
-'''Enzymatic activity:'''
+'''Enzymatic activity:''' none
 <!--none/EC-number ?.?.?.?-->
@@ Line 52: / Line 54: @@
 <!--none/not known/cytotoxic for ''organism name''-->
-'''Safety notes:'''<br>
+'''Safety notes:'''<br> health_risk
 <!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 <!--Known and anticipated security issues: none/other.-->
 '''Intellectual property:'''
 <!--Information on patent situation.-->
 <!--Intellectual property claims made by the authors.-->
 '''Corresponding part author/authors:'''
 <!--https://igem.org/User_Information.cgi?user_id=????/email-->
 ===Source===
-'''Source:'''<br>
+'''Source:'''<br> pYES2, pYES2 Vector kit, company: Invitrogen
-<!--*Commercial system: plasmid name, system name, company name-->
+<!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 <!--*Preexisting BioBrick ?Bba_number?-->
@@ Line 75: / Line 78: @@
 <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
-'''Organism:'''<br>
+'''Organism:'''<br> Designed for E.coli, Yeast
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->