Difference between revisions of "Part:BBa K784019"
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br> | This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br> | ||
| − | Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the | + | Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome. <br> |
| + | More information is available [http://2012.igem.org/Team:Technion/Project/Phage here]. <br> | ||
Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an ''E. coli'' genome (with the matching att-B sequence) between gal and bio operons. <br> | Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an ''E. coli'' genome (with the matching att-B sequence) between gal and bio operons. <br> | ||
Latest revision as of 19:32, 26 September 2012
Phage Lambda fragment 5 - recombination
This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an E. coli genome (with the matching att-B sequence) between gal and bio operons.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4318
Illegal BamHI site found at 543
Illegal XhoI site found at 6069 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4318
Illegal PstI site found at 4576
Illegal PstI site found at 4823
Illegal AgeI site found at 4396 - 1000COMPATIBLE WITH RFC[1000]