Difference between revisions of "Part:BBa K784039:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K784039 short</partinfo>
 
<partinfo>BBa_K784039 short</partinfo>
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===Design Notes===
 
===Design Notes===
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No particular considerations.
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We planed to add T7*(K1F) RNAP primers ,that included tails with restriction site, by PCR : XbaI at the 5' end. There wasn’t any necessity to add the suffix restriction sites at the 3' because it was already at the end of the RNAP.
  
  
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===Source===
 
===Source===
  
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The terminator is from the parts registry (Part:BBa_B0015). RNAP are from: http://nar.oxfordjournals.org/content/early/2012/06/28/nar.gks597.full
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The terminator is from the parts registry (Part:BBa_B0015). RNAP are from:
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Temme K., et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.
  
 
===References===
 
===References===

Latest revision as of 19:19, 26 September 2012

T7*(K1F) RNA polymerase + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We planed to add T7*(K1F) RNAP primers ,that included tails with restriction site, by PCR : XbaI at the 5' end. There wasn’t any necessity to add the suffix restriction sites at the 3' because it was already at the end of the RNAP.


Source

The terminator is from the parts registry (Part:BBa_B0015). RNAP are from: Temme K., et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.

References