Difference between revisions of "Part:BBa K819008:Design"
Zhangzidong (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | Lux CDABEG genes and related RBS from [https://parts.igem.org/Part:BBa_K325909:Design BBa_K325909] are placed under T7 promoter [https://parts.igem.org/Part:BBa_I712074 | + | Lux CDABEG genes and related RBS from luxbrick([https://parts.igem.org/Part:BBa_K325909:Design BBa_K325909]) are placed under T7 promoter ([https://parts.igem.org/Part:BBa_I712074 BBa_I712074]). |
===Source=== | ===Source=== | ||
| − | + | Lux brick (BBa_K325909) and T7 promoter (BBa_I712074) are from 2012 spring distribution kit. | |
| − | T7 promoter | + | |
===References=== | ===References=== | ||
| + | 1. Slock, J.(1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri. Am.Biology.Teacher, 57:225:227<br /> | ||
| + | 2. Meighen, E.A.(1994) Genetics of bacterial bioluminescence. Annu.Rev.Genet., 28:117:139 | ||
Latest revision as of 19:06, 26 September 2012
Lux Operon under T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2012
Illegal XhoI site found at 2842 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726
Design Notes
Lux CDABEG genes and related RBS from luxbrick(BBa_K325909) are placed under T7 promoter (BBa_I712074).
Source
Lux brick (BBa_K325909) and T7 promoter (BBa_I712074) are from 2012 spring distribution kit.
References
1. Slock, J.(1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri. Am.Biology.Teacher, 57:225:227
2. Meighen, E.A.(1994) Genetics of bacterial bioluminescence. Annu.Rev.Genet., 28:117:139