Difference between revisions of "Part:BBa K819017"
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SulA promoter belongs to the SOS regulon family. In <i>E.coli</i> it promotes the transcription of SulA protein. LexA protein in <i>E.coli</i> can bind to SulA promoter, inhibiting the transcription of the genes downstream.<br /> | SulA promoter belongs to the SOS regulon family. In <i>E.coli</i> it promotes the transcription of SulA protein. LexA protein in <i>E.coli</i> can bind to SulA promoter, inhibiting the transcription of the genes downstream.<br /> | ||
However, with mutations in specific sites which LexA protein will bind to, SulA408 promoter will not be bound by LexA protein, but is compatible to LexA408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.<br /> | However, with mutations in specific sites which LexA protein will bind to, SulA408 promoter will not be bound by LexA protein, but is compatible to LexA408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.<br /> | ||
| − | For example, the optimized <i>Luminesensor</i> with a LexA408 domain ([https://parts.igem.org/ | + | For example, the optimized <i>Luminesensor</i> with a LexA408 domain ([https://parts.igem.org/Part:BBa_K819005 BBa_K819005]) constructed by [http://2012.igem.org/Team:Peking Peking 2012 iGEM team] can specifically bind to SulA408 promoter and therefore inhibit the gene expression (e.g. [https://parts.igem.org/Part:BBa_K819006 BBa_K819006] is a testing device containing SulA408 promoter constructed by Peking 2012 iGEM team)<br /><br /> |
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Revision as of 19:00, 26 September 2012
Luminesensor repressible SulA408 promoter
SulA promoter in 408 form.
SulA promoter belongs to the SOS regulon family. In E.coli it promotes the transcription of SulA protein. LexA protein in E.coli can bind to SulA promoter, inhibiting the transcription of the genes downstream.
However, with mutations in specific sites which LexA protein will bind to, SulA408 promoter will not be bound by LexA protein, but is compatible to LexA408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.
For example, the optimized Luminesensor with a LexA408 domain (BBa_K819005) constructed by [http://2012.igem.org/Team:Peking Peking 2012 iGEM team] can specifically bind to SulA408 promoter and therefore inhibit the gene expression (e.g. BBa_K819006 is a testing device containing SulA408 promoter constructed by Peking 2012 iGEM team)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]