Difference between revisions of "Part:BBa K819012"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K819012 short</partinfo> | <partinfo>BBa_K819012 short</partinfo> | ||
| − | LacI mutant | + | LacI mutant protein is placed under SulA408 promoter<br /> |
| + | SulA408 promoter ([https://parts.igem.org/Part:BBa_K819017 BBa_K819017]) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the [https://parts.igem.org/Part:BBa_K819005 optimized <i>Luminesensor</i>] constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in <i>E.coli</i>. | ||
| + | <br />LacI mutant from [https://parts.igem.org/Part:BBa_K395400 BBa_K395400] can repress lac promoter. So this part can work as a connection between two systems. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 18:56, 26 September 2012
Luminesensor repressible LacIM generator
LacI mutant protein is placed under SulA408 promoter
SulA408 promoter (BBa_K819017) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized Luminesensor constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in E.coli.
LacI mutant from BBa_K395400 can repress lac promoter. So this part can work as a connection between two systems.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]