Difference between revisions of "Part:BBa K873003"

Revision as of 18:25, 26 September 2012

conjugative transfer of RFG

This part contains a reporter gene I13521,combined with OriTR(the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.)

Function

Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives
us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency
easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation
rate, conjugation time, and conjugation efficiency between different strains.

How does it works?

Results

The TEM image of conjugation:

The agar Luria-Bertani medium of DH5α: -------------------------------------------------------------- The agar Luria-Bertani medium of BL21:

The agar Luria-Bertani medium of HB101: -------------------------------------------------------------- The agar Luria-Bertani medium of conjugation:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127
Illegal AgeI site found at 1014
Illegal AgeI site found at 1126
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 19

@@ Line 20: / Line 20: @@
 The agar Luria-Bertani medium of DH5α:  --------------------------------------------------------------    The agar Luria-Bertani medium of BL21:
 [[Image:seulfig2.jpg]]                            [[Image:seulfig3.jpg]]
+The agar Luria-Bertani medium of HB101:  --------------------------------------------------------------     The agar Luria-Bertani medium of conjugation:
+[[Image:seulfig4.jpg]]                             [[Image:seulfig5.jpg]]