Difference between revisions of "Part:BBa K873003"

Revision as of 18:23, 26 September 2012

conjugative transfer of RFG

This part contains a reporter gene I13521,combined with OriTR(the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.)

Function

Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives
us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency
easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation
rate, conjugation time, and conjugation efficiency between different strains. 

How does it works?

Results

The TEM image of conjugation:

The agar Luria-Bertani medium of DH5α: -------------------------------------------------------------- The agar Luria-Bertani medium of BL21:

Sequence and Features