Difference between revisions of "Part:BBa K873003"

(→‎Results)
(→‎Results)
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The TEM image of conjugation:
 
The TEM image of conjugation:
 
[[Image:fig1.jpg]]
 
[[Image:fig1.jpg]]
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The agar Luria-Bertani medium of DH5α:      The agar Luria-Bertani medium of BL21:
 
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[[Image:Example.jpg]]                          [[Image:Example.jpg]]
 
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The agar Luria-Bertani medium of HB101:      The agar Luria-Bertani medium of conjugation:
 
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[[Image:Example.jpg]]                          [[Image:Example.jpg]]
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:59, 26 September 2012

conjugative transfer of RFG

This part contains a reporter gene I13521,combined with OriTR(the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.)

Function

Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives
us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency
easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation
rate, conjugation time, and conjugation efficiency between different strains.

How does it works?

Results

The TEM image of conjugation: Fig1.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 127
    Illegal AgeI site found at 1014
    Illegal AgeI site found at 1126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 19