Difference between revisions of "Part:BBa K819005"

Revision as of 17:53, 26 September 2012

Constitutive LuxBrick Generator

Summary

This part serves as an ultra sensitive photoreceptor (which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoters like RecA408 promoter and SulA408 promoter.

It is a fusion protein consisting of the DNA binding domain of repressor LexA originated from E.coli endogenous SOS system, and fungus N.crass light sensing protein VVD which forms a rapidly exchanging dimer when excited by blue light. Both the DNA binding doamin and dimerization domain of this protein were mutated at specific sites for better bio-orthogonality and higher on-off ratio. (to see the design page for details, please click here)

We characterized this luminesensor in different ways and all the results showed that this luminesensor worked quite well. (to see the characterization page for details, please click here)

To learn more details about this luminesensor and the related project by Peking 2012 iGEM team, please visit our [http://2012.igem.org/Team:Peking wiki]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3014
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2012
Illegal XhoI site found at 2842
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726

@@ Line 75: / Line 75: @@
 This part serves as <b>an ultra sensitive photoreceptor</b> (which can sense light as weak as moonlight) and will induce a <b>light-dependent repression</b> of genes under specific promoters like [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 RecA408 promoter] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 SulA408 promoter].
-It is a fusion protein consisting of the DNA binding domain of <i>E.coli</i> endogenous SOS system repressor LexA, and fungus <i>N.crass</i> light sensing protein VVD which forms a rapidly exchanging dimer when excited by blue light. Both the DNA binding doamin and dimerization domain of this protein were mutated at specific sites for better bio-orthogonality and higher on-off ratio.  (to see the <b>design</b> page for details, please click [https://parts.igem.org/Part:BBa_K819005:Designing here])
+It is a fusion protein consisting of the DNA binding domain of repressor LexA originated from <i>E.coli</i> endogenous SOS system, and fungus <i>N.crass</i> light sensing protein VVD which forms a rapidly exchanging dimer when excited by blue light. Both the DNA binding doamin and dimerization domain of this protein were mutated at specific sites for better bio-orthogonality and higher on-off ratio.  (to see the <b>design</b> page for details, please click [https://parts.igem.org/Part:BBa_K819005:Designing here])
 We characterized this luminesensor in different ways and all the results showed that this luminesensor worked quite well. (to see the <b>characterization</b> page for details, please click [https://parts.igem.org/Part:BBa_K819005:Characterization here])