Difference between revisions of "Part:BBa K819005"
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Revision as of 17:39, 26 September 2012
Constitutive LuxBrick Generator
Summary
This part serves as an ultra sensitive photoreceptor (which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoters like RecA408 promoter and SulA408 promoter.
It is a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass light sensing protein VVD. Both the LexA domain and VVD domain of this protein were mutated at specific sites for better bio-orthogonality and higher on-off ratio. (to see the design page for details, please click here)
We characterized this luminesensor in different ways and all the results showed that this luminesensor worked quite well. (to see the characterization page for details, please click here)
To learn more details about this luminesensor and the related project by Peking 2012 iGEM team, please visit our [http://2012.igem.org/Team:Peking wiki]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2012
Illegal XhoI site found at 2842 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726