Difference between revisions of "Part:BBa K733018:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K733018 short</partinfo>
 
<partinfo>BBa_K733018 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Not available at this moment.
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None.
  
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===Source===
  
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We digest and ligate our [https://parts.igem.org/Part:BBa_K733002 ''xylR''+''PxylA''] and [https://parts.igem.org/Part:BBa_E0240 BBa_E0240] from 2012 Kit Plate.
  
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===Source===
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Backbone: ''xylR''+''PxylA'' in pSB1C3. Enzymes used: SpeI and PstI-HF.
  
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Not available at this moment.
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Insert: BBa_E0240. Enzymes used: XbaI and PstI-HF. Purified by gel puficiation.
  
 
===References===
 
===References===

Latest revision as of 17:02, 26 September 2012

xylR+PxylA+RBS+GFP+Double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2058


Design Notes

None.

Source

We digest and ligate our xylR+PxylA and BBa_E0240 from 2012 Kit Plate.

Backbone: xylR+PxylA in pSB1C3. Enzymes used: SpeI and PstI-HF.

Insert: BBa_E0240. Enzymes used: XbaI and PstI-HF. Purified by gel puficiation.

References