Difference between revisions of "Part:BBa K733012:Design"
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===Source=== | ===Source=== | ||
| − | We digest and ligate our xylR+PxylA+RBS+ydcE and | + | We digest and ligate our [https://parts.igem.org/Part:BBa_K733011 ''xylR''+''PxylA''+RBS+''ydcE''] and [https://parts.igem.org/Part:BBa_K733010 ''Ptms''+RBS+''ydcD'']. |
| − | Backbone: | + | Backbone: ''Ptms''+RBS+''ydcD'' in pSB1C3. Enzymes used: EcoRI and XbaI. |
| − | Insert: xylR+PxylA+RBS+ydcE. Enzyme used: EcoRI and SpeI. Purified by gel purification. | + | Insert: ''xylR''+''PxylA''+RBS+''ydcE''. Enzyme used: EcoRI and SpeI. Purified by gel purification. |
===References=== | ===References=== | ||
Latest revision as of 16:42, 26 September 2012
xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 847
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
We digest and ligate our xylR+PxylA+RBS+ydcE and Ptms+RBS+ydcD.
Backbone: Ptms+RBS+ydcD in pSB1C3. Enzymes used: EcoRI and XbaI.
Insert: xylR+PxylA+RBS+ydcE. Enzyme used: EcoRI and SpeI. Purified by gel purification.