Difference between revisions of "Part:BBa K875001"
 
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This promoter is composed by phage T5 promoter and Cumate operator. It is repressed in the presence of CymR protein which binds the Cumate Operator.
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This promoter is composed by a partial phage T5 promoter and Cumate operator. The expression of a downstream gene is repressed by the presence of CymR protein which binds the Cumate Operator. Adding p-cumate to the culture the CymR is inactivated in his repression and the expression of the gene.
  
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[[Image:Cumts3.png|center|500px]]
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[[Image:TriesteT5_CuO_red.jpg|center|500px]]
  
  
  
 
1. The cumate gene-switch: a system for regulated expression in mammalian cells
 
1. The cumate gene-switch: a system for regulated expression in mammalian cells
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Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43   doi:10.1186/1472-6750-6-43 )
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Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 )
  
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2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains                 Young J. Choi, Lyne Morel, Teffanie Le François Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. Míguez (Applied and Environmental Microbiology, Aug. 2010, p. 5058–5066 Vol.76  doi:10.1128/AEM.00413-10)
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2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains Young J. Choi, Lyne Morel, Teffanie Le François Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. Míguez (Applied and Environmental Microbiology, Aug. 2010, p. 5058–5066 Vol.76  doi:10.1128/AEM.00413-10)
  
  

Latest revision as of 16:41, 26 September 2012

T5 Cumate Operator

 This promoter is composed by a partial phage T5 promoter and Cumate operator. The expression of a downstream gene is repressed by the presence of CymR protein which binds the Cumate Operator. Adding p-cumate to the culture the CymR is inactivated in his repression and the expression of the gene.

TriesteT5 CuO red.jpg


1. The cumate gene-switch: a system for regulated expression in mammalian cells Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 )

2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains Young J. Choi, Lyne Morel, Teffanie Le François Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. Míguez (Applied and Environmental Microbiology, Aug. 2010, p. 5058–5066 Vol.76 doi:10.1128/AEM.00413-10)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]