Difference between revisions of "Part:BBa K909007:Design"
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<partinfo>BBa_K909007 short</partinfo> | <partinfo>BBa_K909007 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Deletion of | + | Deletion of alpha 8-10 helices might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracycline |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Moncivais K, Zhang ZJ., Tetracycline repressor-based mammalian two-hybrid systems. Methods in Molecular Biology, 2012, Volume 812, 259-273. | ||
Latest revision as of 16:41, 26 September 2012
TetR DNA binding domain containing BamHI site for protein fusions
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 382
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Deletion of alpha 8-10 helices might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracycline
Source
E. coli Tn10 transposon
References
Moncivais K, Zhang ZJ., Tetracycline repressor-based mammalian two-hybrid systems. Methods in Molecular Biology, 2012, Volume 812, 259-273.