Difference between revisions of "Part:BBa K733010"
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<partinfo>BBa_K733010 short</partinfo> | <partinfo>BBa_K733010 short</partinfo> | ||
| − | This construct contains a low efficient constitutive promoter pTms with an antitoxin gene ydcD (endB). The gene ydcD can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ydcE (ndoA). (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD. | + | This construct contains a low efficient constitutive promoter pTms with an antitoxin gene ''ydcD (endB)''. The gene ''ydcD'' can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ''ydcE (ndoA)''. (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD. |
| − | + | ''Ptms'' promoter: [https://parts.igem.org/Part:BBa_K733001 BBa_K733001] | |
| − | ydcD: [https://parts.igem.org/Part:BBa_K733003 BBa_K733003] | + | ''ydcD'': [https://parts.igem.org/Part:BBa_K733003 BBa_K733003] |
| − | ydcE: [https://parts.igem.org/Part:BBa_K733004 BBa_K733004] | + | ''ydcE'': [https://parts.igem.org/Part:BBa_K733004 BBa_K733004] |
Cell Growth Inhibition Device: [https://parts.igem.org/Part:BBa_K733012 BBa_K733012] | Cell Growth Inhibition Device: [https://parts.igem.org/Part:BBa_K733012 BBa_K733012] | ||
Revision as of 16:32, 26 September 2012
Ptms+RBS+ydcD.
This construct contains a low efficient constitutive promoter pTms with an antitoxin gene ydcD (endB). The gene ydcD can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ydcE (ndoA). (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD.
Ptms promoter: BBa_K733001
ydcD: BBa_K733003
ydcE: BBa_K733004
Cell Growth Inhibition Device: BBa_K733012
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." Molecular microbiology. 56.5 (2005): 1139-1148. Print.